【依据】

——WHO 实验室生物安全手册（第三版），2004（2020年已发布第四版）

第十章 生物安全柜：关于“Open flames”的描述：

Open flames should be avoided in the near microbe-free environment created inside the BSC. They disrupt the airflow patterns and can be dangerous when volatile, flammable substances are also used. To sterilize bacteriological loops, microburners or electric “furnaces” are available and are preferable to open flames.

在生物安全柜内所形成的几乎没有微生物的环境中，应避免使用明火。使用明火会对气流产生影响，并且在处理挥发性物品和易燃物品时，也易造成危险。在对接种环进行灭菌时，可以使用微型燃烧器或电炉，而不应使用明火。

——美国CDC 微生物和生物医学实验室生物安全（第六版），2020

Part 5—BSC Use by the Investigator: Work Practices and Procedures > Operations within a Class II BSC

Part 5—研究者的生物安全柜使用：工作规范和程序 > 二级生物安全柜中的操作

Open flames are neither required nor recommended in the near microbe-free environment of a biological safety cabinet. On an open bench, flaming the neck of a culture vessel will create an upward air current that prevents microorganisms from falling into the tube or flask. An open flame in a BSC, however, creates turbulence that disrupts the pattern of HEPA-filtered air being supplied to the work surface. When deemed absolutely necessary and approved by the appropriate facility authorities after a thorough risk assessment, touch-plate micro burners equipped with a pilot light to provide a flame on demand may be used. Internal cabinet air disturbance and heat buildup will be minimized. The burner must be turned off when work is completed. Small electric furnaces are available for decontaminating bacteriological loops and needles and are preferable to an open flame inside the BSC. Disposable loops should be used whenever possible.

【建议】

综上，不建议使用明火，因明火会影响安全柜内气流组织；对接种环进行灭菌可以选择使用微型电炉；如确需使用明火，需通过风险评估并经设施负责人批准。

不能。影响并破坏气流组织